Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Title:
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
Author:
Chen, Lu
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (524 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol describes a procedure for generating and purifying wild type and mutant versions of the human INO80 chromatin remodeling complex. Epitope tagged versions of INO80 subunits are stably expressed in HEK293 cells, and complete complexes and complexes lacking specific sets of subunits are purified by immunoaffinity chromatography.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51720