Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
Title:
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
Author:
Kučerová-Levisohn, Martina
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (507 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52119