Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
Title:
Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
Author:
Cole, Jennifer M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (710 seconds)
Series:
Medicine
General Note:
Title from resource description page
Abstract:
Cancer stem cells (CSCs) are implicated in tumor relapse due to chemoresistance. We have optimized a protocol for selection and expansion of CSCs from ovarian cancer cell lines. By treating cells with the chemotherapeutic cisplatin and culturing in a stem cell promoting media we enrich for non-adherent CSC cultures.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51891