Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Title:
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Author:
Hartley, Brigham J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (733 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Reporter cell lines offer a means to visualize, track and isolate cells of interest from heterogeneous populations. However, gene-targeting using conventional homologous recombination in human embryonic stem cells is extremely inefficient. Herein, we describe targeting CNS midbrain specific transcription factor PITX3 locus with EGFP using zinc-finger nuclease enhanced homologous recombination.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51764