Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
Title:
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
Author:
Chen, Kevin G.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (507 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present human pluripotent stem cell (hPSC) culture protocols, based on non-colony type monolayer (NCM) growth of dissociated single cells. This new method, utilizing Rho-associated kinase inhibitors or the laminin isoform 521 (LN-521), is suitable for producing large amounts of homogeneous hPSCs, genetic manipulation, and drug discovery.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51519