Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Title:
Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Author:
Park, Sang-Hyuck
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (883 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
A double stranded RNA interference (dsRNAi) technique is employed to down-regulate the maize cinnamoyl coenzyme A reductase (ZmCCR1) gene to lower plant lignin content. Lignin down-regulation from the cell wall is visualized by microscopic analyses and quantified by the Klason method. Compositional changes in hemicellulose and crystalline cellulose are analyzed.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51340