Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Title:
Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Author:
Ertürk, Ali
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (469 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
To obtain high-resolution images of fluorescently labeled cells within large tissues, ideally, the biological samples should be imaged without sectioning. 3DISCO is a straightforward tissue clearing procedure based on sequential incubation with organic solvents. Upon clearing, the organs become transparent allowing an end-to-end laser scan of the specimen.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51382