Super-resolution Imaging of Neuronal Dense-core Vesicles
Title:
Super-resolution Imaging of Neuronal Dense-core Vesicles
Author:
Scalettar, Bethe A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (570 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51394