Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Title:
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
Author:
Tolonen, Andrew C.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (713 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51416