Isolation of Small Noncoding RNAs from Human Serum
Title:
Isolation of Small Noncoding RNAs from Human Serum
Author:
Khoury, Samantha
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (404 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51443