Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Title:
Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Author:
Mazzei, Luca
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (780 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
Isothermal titration calorimetry measures heat flow released or absorbed in chemical reactions. This method can be used to quantify enzyme-catalysis. In this paper, the protocol for instrumental setup, experiment running, and data analysis is generally described, and applied to the characterization of enzymatic urea hydrolysis by jack bean urease.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51487