Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Title:
Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Author:
Milet, Cecile
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (547 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol describes how to dissect premigratory cranial neural crest (NC) from Xenopus laevis neurulas. These explants can be plated on fibronectin and cultured in vitro, or grafted back into host embryos. This technique allows studying the mechanisms of NC epithelium-to-mesenchyme transition, migration, and differentiation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51118