2D and 3D Chromosome Painting in Malaria Mosquitoes
Title:
2D and 3D Chromosome Painting in Malaria Mosquitoes
Author:
George, Phillip
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (597 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Chromosome painting is a useful method for studying organization of the cell nucleus and evolution of the karyotype. Here, we demonstrate an approach to isolate and amplify specific regions of interest from single polytene chromosomes that are subsequently used for two- and three-dimensional fluorescent in situ hybridization (FISH).
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51173