
Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
Title:
Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
Author:
Horstick, Eric J.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (358 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Zebrafish are an emerging system for modeling human disorders of the skeletal muscle. We describe a fast and efficient method to isolate skeletal muscle myofibers from embryonic and larval zebrafish. This method yields a high-density myofiber preparation suitable for study of single skeletal muscle fiber morphology, protein subcellular localization, and muscle physiology.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50259