Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Title:
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Author:
Velapatiño, Billie
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (511 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50730