Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Title:
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Author:
Ying, Wei
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (607 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50323