Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Title:
Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
Author:
McDonald, Marguerite K.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (645 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50294