Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Title:
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
Author:
Keller, Charlotte
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (639 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50116