Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Title:
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Author:
Wenzel, Vera
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (459 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We report a method to isolate naïve multipotent skin-derived precursor (SKP) cells from primary human fibroblast cultures. We show that these SKPs derived from fibroblast cultures share similar stem cell properties to the ones derived directly from human skin biopsies. These cells express the neural crest marker, nestin, in addition to the multipotent markers such as OCT4 and Nanog.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50185