Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
Title:
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
Author:
Zhong, Ling
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (830 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve structures and antigens in the CNS to study and characterize synaptic proteins in rat hippocampal CA1 pyramidal neurons.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50273