A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
Title:
A Simple Chelex Protocol for DNA Extraction from Anopheles spp.
Author:
Musapa, Mulenga
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (436 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
A rapid and affordable way to extract quality malaria parasite and vector DNA from mosquito specimens is described. Capitalizing on chelating properties of Chelex resin, the simple method enables genotyping of malaria parasites in mosquito mid-gut and salivary gland phases, as well as molecular identification of the Anopheles sibling species by PCR.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3281