Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Title:
Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy
Author:
Watanabe, Shigeki
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (793 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3995