Isolation and Primary Culture of Rat Hepatic Cells
Title:
Isolation and Primary Culture of Rat Hepatic Cells
Author:
Shen, Ling
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (426 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3917