Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
Title:
Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
Author:
Shimizu, Saori
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (752 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein isolation.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3257