Isolation of CD133+ Liver Stem Cells for Clonal Expansion
Title:
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
Author:
Rountree, C. Bart
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (726 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3183