Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Title:
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Author:
Chatterjee, Papri
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (624 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3110