Seven Steps to Stellate Cells
Title:
Seven Steps to Stellate Cells
Author:
Maschmeyer, Patrick
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (400 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here we describe a method for the isolation of hepatic stellate cells from mouse liver. For stellate cell purification, mouse livers are digested in situ and in vitro by pronase-collagenase treatment prior to density gradient centrifugation. This technique yields highly pure hepatic stellate cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2710