Flow Cytometry Purification of Mouse Meiotic Cells
Title:
Flow Cytometry Purification of Mouse Meiotic Cells
Author:
Getun, Irina V.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (643 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2602