Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Title:
Analysis of Pluripotent Stem Cells by using Cryosections of Embryoid Bodies
Author:
Gomes, Ismael C.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (414 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Pluripotent stem cells growing in suspension differentiate into embryoid bodies (EBs). Here we demonstrate how to obtain high quality EB cryosections useful for studying cellular and molecular aspects of embryogenesis, while preserving their organization as aggregates.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2344