Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
Title:
Production of Chick Embryo Extract for the Cultivation of Murine Neural Crest Stem Cells
Author:
Pajtler, Kristian
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (612 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
To cultivate neural crest stem cells (NCSC) in vitro, a special medium (NCSCM) is required. Essential part of NCSCM is chick embryo extract (CEE). We here describe accurate techniques to produce a maximized amount of pure and high quality CEE, including details as the isolation, maceration, centrifugation, and filtration processes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2380