Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
Title:
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
Author:
Rasheed, Zeshaan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (704 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2169