Primer-Free Aptamer Selection Using A Random DNA Library
Title:
Primer-Free Aptamer Selection Using A Random DNA Library
Author:
Pan, Weihua
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (674 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2039