Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
Title:
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
Author:
Tremblay, Marie-Eve
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (527 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2021