In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
Title:
In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
Author:
Yamada, Mayumi
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (701 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
In this video, we are showing how to label human embryonic stem cells (hESC) with manganese chloride (MnCl2) which can enter cells via voltage-gated calcium channels when the cells are biologically active. Additionally, we show the use of MnCl2 as cellular MRI contrast agent to determine the in vitro viability of hESC.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/827