Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Title:
Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Author:
P. Matheu, Melanie
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (793 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Antigen presentation in secondary lymphoid organs by dendritic cells is crucial for the initiation of the T cell mediated adaptive immune response. Here we demonstrate the culture of bone marrow derived murine dendritic cells, activation, and labeling for 2-photon imaging.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/773