Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Title:
Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination
Author:
Das, Suchismita
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (925 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Once a gene is identified as potentially refractory for the dengue virus, it must be evaluated for it's role in preventing viral infections within the mosquito. This protocol illustrates how the extent of dengue infections of mosquitoes can be assayed. The techniques for growing up the virus in culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/220