Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
Title:
Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
Author:
Garver, Lindsey
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (708 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/230