Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Title:
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Author:
Xi, Zhiyong
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (850 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/222