Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Title:
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
Author:
McKinney-Freeman, Shannon
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (1326 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/162