Intro -- Title Page -- Contents -- P. Bianchini, A. Diaspro, C.J.R. Sheppard and M. Bouzin - Preface -- Course group shot -- Colin J.R. Sheppard - Imaging in image scanning microscopy: A review -- Barbara Storti and Ranieri Bizzarri - Theory of photochromic Forster energy transfer -- Giuseppe Vicidomini - Single-photon laser-scanning microscopy -- Luca Lanzano, Elena Cerutti, Elisabetta Di Franco, Anna Provvidenza Privitera and Alberto Diaspro - Super-resolution imaging using fluorescence lifetimes and phasor analysis -- M. Mazzocca and D. Mazza - Exploring the eukaryotic nucleus by multimodal single molecule microscopy -- Laura Finzi and David Dunlap - The tethered particle motion and magnetic tweezer techniques: An introduction and some applications -- Antonio Fiore and Giuliano Scarcelli - Biophysical contrast mechanisms of Brillouin microscopy -- Laura Sironi, Mario Marini, Margaux Bouzin, Laura D?Alfonso, Maddalena Collini, Giuseppe Chirico, Claudio Conci, Emanuela Jacchetti, Manuela Teresa Raimondi, Alessandra Nardini, Giulio Cerullo, Rebeca Martinez Vazquez and Roberto Osellame - Laser microfabrication for optical sensing and imaging in vivo -- Peter Saggau - Dynamic diffractive optical elements in microscopy and nanoscopy -- F. Balzarotti - Information theory for single-molecule observation: MINFLUX localization -- Posters -- M. Angelini, F. Floris, F. Marabelli, E. Manobianco and L. Lopez-Sanchez - Gold nanohole arrays: From structural optimization to biosensing applications -- Payvand Arjmand and Marc Guillon - 3D compressive STED microscopy with speckles -- L. Cuneo, S. Civita, P. Bianchini and A. Diaspro - Scattering networks: A tool to remove the background -- A. Passera, M. Geismann, M. Shirzadian, A. Gomez Segalas and F. Balzarotti - DNA-PAINT applied to MINFLUX.

S. Zappone - Fluorescence correlation spectroscopy to determine the role of non-coding RNAs in alpha-synuclein phase transitions -- List of participants.