Metal-Organic Frameworks (MOFs) represent a novel class of highly porous molecular structures, 6-TG loaded on these nanoparticles to maximize therapeutic efficacy and reduce toxicity. MOFs are composed of metal ions or clusters connected by multidentate organic bridging ligands, and because of their appealing properties, they have great potential for application in a range of environments, including ZIF-8 and UiO-66. Consequently, the drug-nanocomplex was generated, leading to the formation of 6-TG@ZIF-8 and 6- TG@UiO-66 nanoparticles. These nanoparticles are cationic, biocompatible, biodegradable, immunogenicity-low, and permeability-enhancing. In addition, bacterial inhibition was investigated using the serial dilution method of 6-TG, 6-TG@ZIF-8 and 6-TG@UiO-66 nanoparticles on the gram-positive bacteria family Staphylococcus aureus (S. aureus) and the gram-negative bacteria family Escherichia coli (E. coli) in order to show the effects of antibiotics, dose-response relationships, and the effects of antibacterial resistance. Furthermore, one of the many in vitro test systems used to evaluate antioxidant activity was the DPPH assay, which was used to identify both synthetic and natural compounds having antioxidant properties.