BODIPYs represent a significant class of fluorescent dyes that are esteemed for their remarkable and versatile fluorescence characteristics. Due to their unique chemical structures, these dyes exhibit strong absorption of light in the UV-visible spectrum and emit fluorescence at longer wavelengths. Their exceptional photostability, favorable quantum yields, ease of synthesis, and adaptability in various constructions contribute to their prominence in the scientific literature. BODIPYs are extensively utilized in critical applications, including bioimaging, flow cytometry, fluorescent labeling, and detection. Heavy atoms within the BODIPY skeleton can inhibit fluorescence by increasing the rate of intersystem crossing. Consequently, the quantum yield experiences a significant reduction, resulting in a loss of BODIPY's essential fluorescence characteristic. However, BODIPY has the capability to specifically react with certain analytes or molecules, which enables the restoration of its fluorescent properties. This intriguing aspect of BODIPY inspired the synthesis of palladium complexes, which have been effectively employed as fluorescence-based detectors for carbon monoxide gas. Carbon monoxide is an odorless, colorless, and highly toxic gas for both humans and animals. Its detection is crucial due to its significantly higher binding affinity to hemoglobin compared to oxygen. Preclinical studies have also shown that carbon monoxide can have anti-inflammatory effects and offer cellular and tissue protection in living organisms when administered in low doses. In this study, carbon monoxide was detected using fluorescence technique, even at very low concentrations of 187 nM. This capability was further extended to living human cells using fluorescence microscopy, highlighting the remarkable potential for carbon monoxide detection in biological contexts.