Protein engineering and design

Title:

Author:

Carey, Paul R.

ISBN:

9780121596408

Publication Information:

San Diego, Calif. : Academic Press, c1996.

Physical Description:

xiv, 361 p. : ill. ; 24 cm.

Contents:

Preface. -- Prediction: -- M.H. Hecht, Strategies for the Design of Novel Proteins. -- J. Novotny, Computer Methods in Protein Modeling: An Overview. -- Production: -- J.R. Rose and C.S. Craik, The Art of Expression: Sites and Strategies for Heterologous Expression. -- V.L. MacKay and T. Kelleher, Methods for Expressing Recombinant Proteins in Yeast. -- T. Vernet and R. Brousseau, In Vitro Mutagenesis. -- Characterization: -- G.M. Clore and A.M. Gronenborn, Determination of Structures of Larger Proteins in Solution by Three- and Four-Dimensional Heteronuclear Magnetic Resonance Spectroscopy. -- D. Ringe and G.A. Petsko, A Consumer's Guide to Protein Crystallography. -- P.R. Carey and W. Surewicz, Spectroscopic and Calorimetric Methods for Characterizing Proteins and Peptides. -- Applications: -- A. Nathan and J. Kohn, The Design of Polymeric Biomaterials from Natural Alpha-L-Amino Acids. -- K.E. McLane, S.J. M.Dunn, A.A. Manfredi, B.M. Conti-Tronconi, and M.A. Raftery, The Nicotinic Acetylcholine Receptor as a Model for a Superfamily of Ligand-Gated Ion Channel Proteins. -- Chapter References. -- Subject Index.

Strategies for the design of novel proteins / Computer methods in protein modeling: an overview / The Art of expression: sites and strategies for heterologous expression / Methods for expressing recombinant proteins in yeast / In vitro mutagenesis / Determination of structures of larger proteins in solution by three- and four-dimensional heteronuclear magnetic resonance spectroscopy / A Consumer's guide to protein crystallography / Spectroscopic and colorimetric methods for characterizing proteins and peptides / The Nicotinic acetylcholine receptor as a model for a superfamily of ligan-gated ion channel proteins

Abstract:

The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. Key Features * Introduces and defines the techniques involved in protein engineering and design * Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides.

Subject Term:

Protein engineering.

Proteins -- chemistry.

Models, Molecular.

Technologie des protâeines.

Genre: