This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features * Includes a glossary of over 200 terms important for understanding molecular biology * Uses an inexpensive source of eukaryotic cells - great for schools on a budget * Includes Methods Locator that provides instant access to the latest methods * Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques * Phage titration * Gel electrophoresis of DNA * Restriction enzyme digestion * Plasmid isolation * Transformation of E. Coli * Recombinant DNA cloning * Nick translation labeling * Nonradioactive primer labelling * Nonradioactive DNA detection * Southern blotting * Colony hybridization * Purification of plant DNA * RNA purification * Northern blotting * Purification of poly A+ RNA * Polymerase chain reaction (PCR).