Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Title:
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Author:
Ahn, Jeeyoon Jennifer
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (610 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54554