Laser-guided Neuronal Tracing In Brain Explants
Title:
Laser-guided Neuronal Tracing In Brain Explants
Author:
Albrecht, Otto
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (400 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
We describe a technique to label neurons and their processes via anterograde or retrograde tracer injections into brain nuclei using an in vitro preparation. We modified an existing method of in vitro tracer electroporation by taking advantage of fluorescently labeled mouse mutants and basic optical equipment in order to increase labeling accuracy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53333