Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Title:
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Author:
Aw, Jong Ghut Ashley
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (692 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55255