Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
Title:
Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles
Author:
Baedke, Paige E.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (475 seconds)
Series:
Environment
General Note:
Title from resource description page
Abstract:
In reptiles, skin lipids from conspecifics are crucial for sexual signaling, with potential use in invasive species management. Here, we describe protocols for extracting skin lipids from shed skin or whole animals, determining and analyzing the total lipid mass, and separating the lipids using fractionation via column chromatography.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59018