Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Title:
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Author:
Baker, Mark A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (570 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Proteomic analysis of any cell type is highly dependent on both purity and pre-fractionation of the starting material in order to de-complexify the sample prior to liquid chromatography mass spectrometry (MS). By using back-flushing techniques, pure spermatozoa can be obtained from rodents. Following digestion, phosphopeptides can be enriched using TiO2.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/51546