Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
Title:
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
Author:
Bandopadhyay, Rina
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (567 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53415